Elucidating the glycan-binding specificity and structure of Cucumis melo agglutinin, a new R-type lectin

• Jon Lundstrøm,
• Emilie Gillon,
• Valérie Chazalet,
• Nicole Kerekes,
• Antonio Di Maio,
• Ten Feizi,
• Yan Liu,
• Annabelle Varrot and
• Daniel Bojar

Beilstein J. Org. Chem. 2024, 20, 306–320, doi:10.3762/bjoc.20.31

• groundwork for further exploration of its biological and therapeutic potential. Keywords: carbohydrate; glycan array; melon; plant lectin; R-type; Introduction Lectins have long been the subject of intense scientific scrutiny, serving as molecular bridges that span the realms of biochemistry, cellular

• member of characterized melon lectins, namely the Cucumis melo agglutinin (CMA1), an R-type lectin derived from melon. Prior to our study, CMA1 was only a predicted protein from genomic sequencing, with moderate certainty scores on lectin-specific databases. Our comprehensive analysis using glycan array

• answer the question whether CMA1 was a functional lectin and, if yes, what its binding specificity was. The standard approach to elucidate lectin binding specificity is via glycan array experiments. Here, tagged soluble lectin is added to, often, immobilized glycans and bound lectin is quantified via

GlycoBioinformatics

• Kiyoko F. Aoki-Kinoshita,
• Frédérique Lisacek,
• Niclas Karlsson,
• Daniel Kolarich and
• Nicolle H. Packer

Beilstein J. Org. Chem. 2021, 17, 2726–2728, doi:10.3762/bjoc.17.184

• paper by Mehta et al. [5], who summarize recent developments and available online resources for glycan array data, a very powerful technique for understanding the structural element(s) of glycans required for different lectin binding. This further emphasizes the role of glycans as mediators of cellular

Tools for generating and analyzing glycan microarray data

• Akul Y. Mehta,
• Jamie Heimburg-Molinaro and
• Richard D. Cummings

Beilstein J. Org. Chem. 2020, 16, 2260–2271, doi:10.3762/bjoc.16.187

• ://www.functionalglycomics.org/glycomics/publicdata/primaryscreen.jsp. Description: The Consortium for Functional Glycomics (CFG) database of glycan array data (Figure 3A) is one of the largest archival resources for glycan microarray data. The CFG was founded in 2001 and was funded for about 10 years, during which time the

• include storage of glycan array data from different array formats. This includes the results along with any array-specific metadata such as experiment protocol, array geometry etc. CarbArrayART will offer presentation of data with filtering and sorting functions, and generation of reports. More recently

• , glycan array data was shared only as excel files which are non-interactive and often troublesome to visualize glycan structure alongside data visualizations such as bar charts. Yet manual data analysis is still widely used to deduce most information based on glycan microarray data. The GLycan Array

Automated glycan assembly of arabinomannan oligosaccharides from Mycobacterium tuberculosis

• Alonso Pardo-Vargas,
• Priya Bharate,
• Martina Delbianco and
• Peter H. Seeberger

Beilstein J. Org. Chem. 2019, 15, 2936–2940, doi:10.3762/bjoc.15.288

• lipoarabinomannan (LAM) analogs in urine samples using the Alere lateral flow urine (LF)-LAM assay [13]. However, the low sensitivity of the Alere assay limits its scope. Glycan array screening of more than 100 monoclonal antibodies against more than 60 synthetic glycans related to the mycobacterial cell wall

Total synthesis of a Streptococcus pneumoniae serotype 12F CPS repeating unit hexasaccharide

• Peter H. Seeberger,
• Claney L. Pereira and
• Subramanian Govindan

Beilstein J. Org. Chem. 2017, 13, 164–173, doi:10.3762/bjoc.13.19

• glycosylation of the liberated hydroxy groups. Formation of the β-mannosazide glycoside containing a protected C5 amino linker that serves in the final product as an attachment point for glycan array surfaces or carrier proteins was central to the assembly of trisaccharide 3. To avoid a challenging and often

DNA display of glycoconjugates to emulate oligomeric interactions of glycans

• Alexandre Novoa and
• Nicolas Winssinger

Beilstein J. Org. Chem. 2015, 11, 707–719, doi:10.3762/bjoc.11.81

• designed to emulate HIV's gp120 epitope. Combinatorial assembly and selection of two PNA glycoconjugate libraries on DNA templates. DNA display of ligand bridging opposing binding sites in a lectin (ECL). A glycan array prepared by hybridization of glycan–DNA conjugates and screening of RCA120. Multivalent

The use of glycoinformatics in glycochemistry

• Thomas Lütteke

Beilstein J. Org. Chem. 2012, 8, 915–929, doi:10.3762/bjoc.8.104

• with glycans from the GlycoMinds Ltd. seed database. Primary data of MS experiments and glycan array screens are also available via the CFG website. If a glycan structure has been detected to be bound by a glycan-binding protein in a CFG glycan array experiment, links to the corresponding protein pages

• arrays [4]. CFG Glycan Binding Proteins DB and Lectin Frontier Database store data of glycan array experiments and thus also provide information on the glycan specificity of GBPs. Glycan array data from these two resources and from other research groups are collected and available via a common interface

• for the analysis and conversion of glycan sequence data are summarized in Table 3. The Glycan Fingerprints approach to calculate the degree of diversity in a set of glycan structures is a useful tool to, e.g., evaluate the glycans that are present on a glycan array [79]. GlycanBuilder [80] and